Droplet microfluidic high-throughput screening analysis system

• 详细介绍
• 详细参数

• 详细介绍

  Product introduction:
  

   STYX is a complete solution for any laboratory that customers want to adopt an advanced droplet microfluidic workflow. This platform can realize various applications of droplet cell counting and on-demand operation. STYX combines fluorescence analysis and droplet processing systems with easy-to-use software, making it the perfect solution for functional screening workflows.

  Main technical features of STYX droplet microfluidic high-throughput screening analysis system:

  √ Up to 4 independent lasers (405/488/561/633) can be configured

  √ 4 independent fluorescence detection channels

  √Dual high-speed microscopes can be set for process analysis of slides

  √Inline droplet analysis based on robot vision technology

  √Unlimited chips (compatible with various elastomer chips)

  √Integrated high voltage pulse generator

  √ Embedded user interface software

  √ Small footprint

  
  

  The Styx platform provides an easy-to-use framework for droplet-based functional screening and analysis. Compared with traditional plate-based technology, the platform's screening throughput has increased by >1000 times.

  
  

  Technical principle:

  Single-cell RNA-seq and single-cell ATAC-seq technologies are widely used to create cell type maps for a wide range of organisms, tissues, and disease processes. In order to increase the scale of these atlases, reduce costs, and allow more professional multi-group inspections, customized droplet microfluidics can provide complementary solutions for commercial installations.

  We have developed HyDrop, a flexible general-purpose droplet microfluidic platform, which contains three solutions.

  The first option is to use custom-made oligomers to make soluble hydrogel beads, which can be released in water droplets.

  In the second solution, we use these beads for HyDrop-ATAC in droplets, which is a low-cost protocol. After verifying HyDrop-ATAC, we applied it to the rapid freezing of mouse cortex and generated 8502 high-quality single-cell chromatin accessibility profiles in one run.

  In the third scheme, we use the reaction chemistry and capture sequence of barcode hydrogel beads to capture mRNA. Compared with the previous sCRNA-seq analysis based on open source water droplets (Drop-seq and inDrop), it has significant throughput and sensitivity. improve. HyDrop is currently able to generate single-cell data with high throughput and lower cost. We expect HyDrop to be further developed and compatible with new (multi)omics protocols.

  
  

  Application areas:

  ●Single cell analysis

  ●Cell sorting

  ●Cell sequencing

  ●Drug screening

  ●Synthetic biology

  ●Antibody discovery

  ●Directed evolution

  ●Astrobiology

  
  

  Applications

  Single cell analysis:

  Single-cell analysis of the immune microenvironment of breast tumors, coupled with computational analysis, generated an immune map of breast cancer, indicating the continuous T cell activation and differentiation status.

  
  

  Achieved:

  Single-cell RNA-seq shows expansion of immune cell phenotype in tumor

  Biscuit identifies cell population patterns that differ in co-expression

  T cells reside on a continuous trajectory of activation and differentiation

  Combining environmental input and TCR use to shape T cell phenotype

• 详细参数

  STYX specifications:

  1. Pressure pump: Type: piezoelectric independent channel: 4; pressure range: 0.5 -25 kpa; pressure change response time: <2.5s; sample storage tank: compatible with standard 1.5ml. 5ml and 15ml laboratory test tubes.

  2. Laser: up to 4 independent wavelengths of 488nm, 405nm, 561nm, 633nm, laser power: 0-20mw. Detection sensitivity: 100us-100ms continuous light pulse, lower limit of photon flux of lohoton/μs at 25℃. Measurement throughput: >1000 samples/sec. Trigger delay: <lo microseconds.

  3. High-speed cameras: 2 independent high-speed cameras, resolution: 1440 x1080, exposure time: 45 us-2ms, recording frame rate: 10-5000 frames per second (depending on the size of the area), video playback rate: 1- 30 Frames/sec.

  4. High-voltage pulse generator: pulse amplitude range: 100-1000Vpp, output cycle: up to 50%, automatic frequency tuning, pulse duration: 100μs-100ms, pulse synchronization delay: 100μs-10ms, error 10μs.

  5. Integrated microscope: independent observation area: 2, optical magnification: 0.7um/px (observation area 1), 1.5um/px observation area 2) Light source: 0-0.25W LED 760 nanometer monochromatic, microfluidic chip size : 25 x 75mm. Stage: motorized 3 direction stage (XYZ).

  6. Software system: GPU: 512−core NVIDIA Volta, 32 TOPs, CPU: 8−core NVIDIA Carmel Arm®v8.2 64−bit CPU8MB L2 + 4MB L3, storage capacity: 32 GB 256−bit LPDDR4× 136.5GB/ s, Chip and droplet analysis rate: <2.0s, Droplet size precision: ±5µm (for nominal 100µm size).

• 产品特色

  因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。

• 详细介绍

  AutoCal系统提供在线的机器人校准方案，旨在快速和自动地保证机械设备的工作性能。因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。这意味着不用猜测哪里会出错，不用浪费宝贵时间在机器人程序重复校准上，产品品质无任何损失。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。