Netherland LSM Automatic high-throughput zebrafish embryo microinjection system

• 详细介绍
• 详细参数

• 详细介绍

  Overview:
  

       LSM is the world's first truly high flux automatic zebrafish micromanipulation injection device, the birth of it can completely satisfy the demand for high-throughput embryos or cell injection and liberation experiment operator's hands, let the microinjection become more simple, rapid, accurate!

  LSM System technical characteristics：

  √  High throughput, 2500 embryos per hour, fully automatic without manual injection

  √ Quantitative injection, high repeatability, convenient for comparative study

  √  Rapid detection typically takes three to seven days, rather than weeks using other technologies

  √ Accurate and automatic 3D positioning, convenient and fast, can achieve efficient automatic imaging (compatible with confocal microscope, etc.)

  √Cultured cells can be spherical in 3D and cultured using cell lines or progenitor cells

  √The tumor microenvironment can be studied. Spheroids can introduce environments into preformed microcrystals, such as cells mixed with collagen

  √Studies of intercellular interactions, such as cancer and immune cells, bacteria and immune cells                

  
  

  Application of zebrafish embryo microinjection：

  1. High-throughput zebrafish embryo injection: the agarose gel is injected into the mold to form an agar-agar grid used to align zebrafish embryos. For injection, different sizes of mesh can be selected according to different sample batches required by the experiment, such as 2580 mesh, 1024 mesh and 9x 100 mesh. It takes less than five minutes to put an embryo into a mold. The quality of the injection is guaranteed by our machines at the closest point to the surface of the fertilized egg and yolk.

  2. 3D cell culture, cell globules: first, collagen, or another hydrogel, is sucked into a porous plate and allowed to be set up. A single cell suspension was then prepared from cell culture or primary tissue. The suspension is mixed with an inert polymer, the PVP, and loaded into a needle. Finally, the cells suspended in a predetermined position are injected into the hydrogel. A typical test is incubated for 3 to 7 days, which can be pathologically treated with standard/confocal microscopy, or sampled by flow cytometry. The reproducible injection position allows imaging of all holes in the same position. The size of the sphere (~300 centile, ~10k cells) and the outer growth of the sphere are replicable, making it an option for active screening for migration, proliferation, etc.

  3. Cell injection: the injection is done at precise locations, so different cell types can be injected at reproducible distances. These injections can be administered at different times to accommodate different growth rates or time-dependent biological interactions. A lot of mixed culture analysis is developing.

• 详细参数

  LSM specification：

  1.Size, Weight：LBH = 50 cm x 100 cm x 50 cm, 50 KG

  2.Available batch size：100, 1024, 2580 个（embryo）

  3.Needle calibration time：45 seconds

  4.Embryo placement time：3 minutes

  5.Injection time：2000 eggs / hour, 1.7 seconds per injection

  6.Instruction time：4 hours

  7.Pressure range:4000 hPa - 8000 hPa，60 PSI - 120 PSI

  8.Connection:G 1/4

  9.Compressed gas source: compressor, compressed gas cylinder,internal compressed air supply

  Compressed gas: compressed air,nitrogen

• 产品特色

  因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。

• 详细介绍

  AutoCal系统提供在线的机器人校准方案，旨在快速和自动地保证机械设备的工作性能。因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。这意味着不用猜测哪里会出错，不用浪费宝贵时间在机器人程序重复校准上，产品品质无任何损失。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。