CellWave Sorter

• 详细介绍
• 详细参数

• 详细介绍

  Technical advantages

  1. No damage: Cell sorting is carried out in a sonic manner, with the sorting process maintaining low shear force and keeping the cells in their natural state.

  Especially suitable for sensitive samples such as stem cells, exosomes, and primary cells.

  2. Ultra-high purity and recovery rate: By precisely manipulating the cells with the sound wave standing field, the sorting purity is >95% and the recovery rate is >80%.

  Far more gentle than conventional flow cytometry sorting (FACS) or magnetic bead sorting (MACS).

  3. High throughput, automation: Processing speeds up to 10^6 cells per minute, supporting continuous sorting and reducing human operational errors.

  Integrated intelligent software with one-click parameter setting for easy repeatability experiments.

  4. Flexible compatibility with a variety of applications: Compatible with a wide range of sample types including blood, tissue dissociation fluid, cultured cells, etc.

  Modular design supports customized sorting strategies (such as sorting by size, density, mechanical properties).

  Technical principles

  CellWave uses high-frequency ultrasonic waves (MHz level) to create a stable Acoustic standing wave field within the microfluidic chip, and manipulates cells or particles without contact through Acoustic Radiation Force:

  1. Acoustic standing wave field: Excited by piezoelectric transducers to form periodic high/low voltage nodes within the microchannel.

  2. Acoustic radiation force: Cells in the acoustic field are driven by the pressure difference to migrate and arrange in an orderly manner towards the acoustic pressure nodes (or inverse nodes, depending on cell characteristics).

  3. Sorting mechanism: Cells of different sizes, densities, or mechanical properties are affected differently by the acoustic force, which enables precise separation.

  Operating Procedures

  1. Sample injection: The cell suspension passes through the microfluidic channel to maintain laminar flow.

  2. Sound field regulation: High-frequency ultrasound activation creates a standing wave field, and cells gather at the sound pressure nodes.

  3. Sorting output: Target cells: Offset to the collection outlet under the effect of acoustic force. Non-target cells: Discharged along the waste liquid stream.

  4 Gentle recovery: The sorted cells remain highly active and can be used directly downstream for culture or analysis.

  Core application areas

  1. Cell therapy and regenerative medicine: Stem cell sorting (such as CAR-T, iPSC preparation) to ensure the safety and consistency of clinical-grade cell products.

  2. Tumor research and liquid biopsy: Efficient enrichment of circulating tumor cells (CTCS) and exosomes to facilitate early cancer screening and efficacy monitoring.

  3. Immunology and infectious diseases: Sorting of rare immune cells (such as antigen-specific T cells) to accelerate vaccine development and immune response research.

  4. Microbiology and Synthetic Biology: High-throughput sorting of bacteria and yeast, optimizing strain screening and biomanufacturing processes.

  Comparative advantages of sorting techniques

  Parameters	CellWave sound wave sorting	Conventional Flow Sorting (FACS)	Magnetic bead sorting (MACS)
  Cell damage	no	Laser/voltage damage cells	Antibody labeling may activate the cells
  Labeling requirements	Not required (can be marked if the sample has special requirements)	Fluorescently labeled	Magnetic bead labeling is required
  Flux	High (continuous flow)	High (Single cell detection)	Low (Batch processing)
  Cost-effectiveness	Low initial purchase cost and simple later maintenance	The initial purchase cost is high, and the later maintenance cost of the laser is high	The initial purchase cost is high, and the later use cost of the magnetic beads is high

  
  

• 详细参数

  Technical parameters

  Classification	Parameters (English)	Parameters (Chinese)	Specifications
  Optical system	Laser	Laser wavelength	488 nm
  	Fluorescence Filter	Fluorescence filter	530/43 nm
  	Detectors	Detector	FITC (Fluorescence), FSC (forward scattering)
  Fluid systems	Sample input
  	Maximum Input Sample Volume	Maximum sample volume	600 µL
  	Minimum Input Sample Volume	Minimum sample volume	150 µL
  	Recommended Input Volume	Recommended sample volume	150-300 µL
  	Recommended Concentration	Recommended sample concentration	0.5-1.5×10⁶ cells/mL
  	Maximum Sheath Volume	Maximum sheath volume	1.2 mL
  	Max Sheath Pressure	Maximum sheath fluid pressure	5 PSI
  	Recommended Sheath Pressure	Recommend sheath pressure	2.25 PSI
  Output and Performance	Output Containers	Output containers	2×1.5 mL EP tubes
  	Throughput	Flux	10,000 cells/sec
  	Max Sample Pressure	Maximum sample pressure	2 PSI
  	Recommended Sample Pressure	Recommended sample pressure	1 PSI

  
  

• 产品特色

  因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。

• 详细介绍

  AutoCal系统提供在线的机器人校准方案，旨在快速和自动地保证机械设备的工作性能。因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。这意味着不用猜测哪里会出错，不用浪费宝贵时间在机器人程序重复校准上，产品品质无任何损失。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。