CellenONE automatic single cell sorter

• 详细介绍
• 详细参数

• 详细介绍

  Technical features:

  
  

  
  

  
  

  
  

  
  

  √Based on gentle and highly accurate piezoelectric acoustic distribution technology

  
  

  √Automatic optical monitoring of the cells in the dispenser nozzle

  
  

  √According to each sample, the machine maps the distribution steps

  
  

  √Single cells after distribution have high activity

  
  

  √The location of the cell determines whether the single cell condition is met, and thus determines whether to be sorted in the next drop

  
  

  
  

  
  

  
  

  
  

  Technical principle:

  
  

  
  

  
  

  
  

  
  

  
  

  
  

  
  

  We have developed a very simple process. Using our glass capillary-based PDC technology, a small amount of micron cell suspension can be sucked with almost no dead volume. Then, use patented dispensing/imaging technology to monitor the dispensing of each drop of liquid medicine. Our PDC dispensing technology usually dispenses a droplet volume of 100-300 picoliters. Since each droplet will be ejected, we monitor it for the presence of a single unit. If a cell exists, we store it in the target vessel of your choice. It may be a micro unit, a micron plate, a slide or other things you like. If there are no cells in the droplet, or there are multiple cells, we will recycle the droplet to ensure that your precious rare cells are not wasted.

  
  

  
  

  
  

  Application areas:

  
  

  ü Cell sorting

  
  

  üSingle cell collection

  
  

  ü Cell clonal expansion

  
  

  ü Cell tissue specificity research

  
  

  üNGS omics analysis

  
  

  Application case

  
  

  HuBBBTM technology:

  
  

  HuBBBTM technology is a very powerful B cell immortalization system, but only 10% of immortalized B cells are successfully cloned, so the data is still very limited. The combination of HuBBBTM and CELLEN ONE significantly increases the cloning efficiency to 90%, saving a lot of time and ensuring the identification of the best clones.

  
  

  
  

  
  

  Cloning field:

  
  

  Purpose: To demonstrate the ability to clone cells for mAb development (e.g. CHO, hybridoma)

  
  

  Difficulty: Ensure the monoclonality and viability of cells isolated and cultured individually

  
  

  CellenONE method: Print single cells on a microscope slide, verify monoclonality, clone recovery/survival by distributing single clones into 96-well plates, and counting n colonies/survival after 7 days.

  
  

  
  

  
  

  
  

  
  

  
  

  
  

  FACS vs CellenONEX1:

  
  

  1. Comparison purpose: compare the clone recovery of BDfacsaria 3 and CellenONEX1

  
  

  2. Experimental cells: CHO cell transformation with GFP-negative reporter gene and drug resistance gene fusion-related genes. During the experiment, 70% of CHOs were GFP negative.

  
  

  3. Experimental details: The cloning is carried out in a 96-well plate, and each well is supplemented with 200μl medium with adrenaline selective antibiotics. After culturing for 5 days, the number of wells with at least 5 cells in a single colony was positive. (GFP+ clones were eliminated due to selective existence)

  
  

  
  

  
  

  4. Expected result:

  
  

  i. The success rate after FACS classification is 100% (excluding GFP +ve)

  
  

  ii. The success rate of CellenONE sorting is 70%.

  
  

• 详细参数

  CellenONE technical parameters:

  
  

  1. Dispensing technology: piezoelectric sound drop on demand

  
  

  2. Dispensing volume: 50-800 pL per drop

  
  

  3. X/Y linear drive, Z axis drive

  
  

  4. Resolution: 1μm

  
  

  5.Accuracy (absolute position): <10μm<>

  
  

  6.Precision (repeat position): <3μm<>

  
  

  7. The fastest speed: 100 cm/sec

  
  

  8.Spottable area (mm): x = 180; y = 120 (2 microtiter plates)

  
  

  9. Dimensions LxWxH (mm): 740 x 750 x 1580, weight 205 kg

  
  

  10.Voltage: 110 V; 220 V

  
  

  
  

  
  

• 产品特色

  因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。

• 详细介绍

  AutoCal系统提供在线的机器人校准方案，旨在快速和自动地保证机械设备的工作性能。因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。这意味着不用猜测哪里会出错，不用浪费宝贵时间在机器人程序重复校准上，产品品质无任何损失。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。