Fox Flash Oxidation Protein Footprinting System

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• 详细介绍

  Product Overview
  The Fox™ Photochemical Protein Footprinting System is a superior, compact, cost-effective, and safe solution for hydroxyl radical protein footprinting (HRPF). It replaces expensive, complex, and hazardous laser-based platforms with our proprietary Fox™ desktop rapid oxidation system.
  To address challenges in reproducibility, GenNext developed the Fox Radical Dosimeter—the world’s first system for measuring OH radicals. It automatically regulates the generation of effective OH radicals in the presence of unpredictable and varying background scavengers. The dosimeter enables real-time measurement of OH radical concentration, simplifying the assessment of biopharmaceutical solutions or experimental conditions prior to MS analysis. This significantly enhances HRPF usability while ensuring labeling credibility and fidelity.
  To eliminate today’s bottlenecks in bioinformatics and data processing, GenNext introduced FoxWare™, a unique software suite specifically designed to automate and accurately process data for HRPF higher order structure (HOS) analysis.
  Unique Advantages of the Fox System
  Direct Assessment of Solvent Accessibility
  Insights gained from studying various classes and systems of proteins using the Fox footprinting method can significantly influence decision-making at every stage of the drug discovery and development process—ultimately enabling the design and production of safer, more effective, and more stable biologics.
  Advanced Structural Validation
  The Fox system validates higher order structure (HOS) of complex biologics (such as monoclonal antibodies) by confirming correct folding and assembly. By comparing experimental Fox data with expected solvent accessibility derived from known protein structures, researchers can assess structural integrity and accuracy of biotherapeutics.
  High Spatial Resolution
  The Fox system offers high spatial resolution data about protein structure. It can precisely identify specific amino acids and regions that are solvent-exposed or buried. This level of detail allows researchers to map protein surfaces and accurately identify regions involved in protein–protein or protein–ligand interactions.
  Versatility Across Protein Classes
  The Fox system accommodates a wide range of protein classes, including soluble proteins, membrane proteins, and protein complexes. It is not limited by protein size, type, or structure, enabling the study of diverse systems. Its ability to handle very large and heterogeneous samples provides deep insights into their structure and functional dynamics.
  Complement to Other Techniques
  The Fox system complements other structural biology methods such as X-ray crystallography, NMR spectroscopy, hydrogen/deuterium exchange (HDX), and cryo-electron microscopy (cryo-EM). It provides information on solvent accessibility and protein dynamics in native solution-state conditions—data often inaccessible through other methods. When combined with these techniques, Fox-HRPF offers a more comprehensive understanding of protein structure and function.
  New Insights Enabled by the Fox System
  Protein–Protein Interactions
  The Fox® system identifies interaction sites and interfaces in protein–protein complexes. By measuring changes in solvent accessibility at interface residues upon binding, it can precisely locate the regions or domains responsible for interaction. This is particularly useful for studying interactions involving monoclonal antibodies and their antigens or other binding partners.
  Epitope Mapping
  Fox provides valuable information about solvent accessibility of residues involved in antigen–antibody interactions and reliably detects changes upon binding. This enables rapid identification and mapping of epitopes on monoclonal antibodies, offering insights into the binding interface and recognition regions. Such information is crucial for the design and optimization of therapeutic antibodies and diagnostic reagents.
  Characterization of Protein–Ligand Interactions
  The Fox system delivers key information on protein–ligand binding. By evaluating changes in solvent accessibility upon ligand binding, it helps identify residues at the binding site and reveals ligand-induced conformational changes. These insights support understanding of binding mechanisms and guide rational drug design.
  Protein Folding & Conformational Changes
  The Fox system detects conformational dynamics within proteins. By comparing Fox data across different conditions or time points, researchers can pinpoint structural rearrangements, as well as exposure or shielding of residues. This enables studies of conformational changes due to ligand binding or environmental factors.
  Allosteric Region Identification
  Fox identifies allosteric regions that are critical for understanding how proteins signal and undergo functional transitions. By revealing these regulatory sites, Fox-based HRPF facilitates elucidation of functional mechanisms and supports drug discovery targeting allosteric hotspots.
  Glycoprotein Analysis
  The Fox system reveals solvent accessibility in glycoproteins. Glycans can shield specific amino acids from oxidation, providing insights into glycan–protein interactions. Additionally, the method aids in understanding conformational shifts under various conditions and the structural basis of glycoprotein function.
  
  Figure 1: HRPF Principle Illustration — Fast Photochemical Oxidation of Proteins (FPOP)
  In FPOP, proteins are mixed with hydrogen peroxide and exposed to pulsed UV light, which photolyzes H₂O₂ into •OH radicals that oxidize solvent-exposed amino acids. After labeling, samples are quenched in a solution containing catalase and methionine, digested, and analyzed using high-resolution LC-MS/MS.
  Application Areas
  Monoclonal Antibodies
  Epitope Mapping
  Paratope Mapping
  Aggregation Studies
  Formulation Research
  Biosimilar Development
  Originator Comparison
  Drug Target Discovery
  Drug Binding Site Characterization
  Allosteric Effects
  Biomolecular Interactions
  Protein–Ligand
  Protein–Protein
  

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  AutoCal系统提供在线的机器人校准方案，旨在快速和自动地保证机械设备的工作性能。因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。这意味着不用猜测哪里会出错，不用浪费宝贵时间在机器人程序重复校准上，产品品质无任何损失。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。