Serial Two-Photon Tomography Whole-Tissue Imaging System

• 详细介绍
• 详细参数

• 详细介绍

  Product Overview
  The Automated Serial Two-Photon 3D Whole-Tissue Imaging System is an automated research platform designed for three-dimensional imaging and spatial analysis of intact mouse brains and large-volume tissue specimens. The system uses a precision microtome to serially section fixed tissue and performs two-photon fluorescence imaging immediately after each section is generated. Hundreds or thousands of sequential images can then be automatically registered, stitched, and reconstructed into a high-resolution 3D dataset, providing comprehensive spatial information ranging from individual cells and neural fibers to large-scale brain structures.
  Unlike conventional two-photon microscopes, which typically focus on localized regions of tissue, the system is designed to image entire brains or large tissue volumes as integrated 3D specimens. Key applications include neuronal and neural-circuit tracing, brain-region connectivity analysis, vascular mapping, neurodegenerative disease research such as Alzheimer's disease, and whole-brain biodistribution studies for AAV and gene therapies. The serial tissue sections can also be preserved for immunohistochemistry, histology, and molecular analysis, allowing these conventional tissue-based results to be correlated with their original three-dimensional spatial locations.
  Product Advantages
  Whole-Brain 3D Imaging: Enables high-resolution 3D imaging of intact mouse brains and large tissue volumes.
  Automated Serial Sectioning: Integrates automated sectioning and two-photon imaging in a continuous section–image workflow.
  High-Resolution Spatial Information: Reveals 3D spatial relationships among cells, neural fibers, blood vessels, and brain structures.
  Tissue Preservation: Serial sections can be retained for subsequent immunohistochemistry and histological analysis.
  Whole-Brain Applications: Particularly suitable for neural circuits, brain diseases, and AAV/gene therapy biodistribution studies.
  Main Product Features
  Multichannel Fluorescence Imaging: Enables detection and analysis of multiple fluorescent labels.
  Precision Microtome: Uses a dedicated high-precision sectioning mechanism for stable and uniform serial tissue sectioning.
  Automated Image Acquisition: Integrates sectioning and imaging for continuous and unattended data collection.
  Large-Scale Data Processing: Supports registration, stitching, and 3D reconstruction of large volumes of sequential images.
  Flexible Sample Handling: Suitable for fixed tissue samples of different sizes and types.
  3D Visualization and Analysis: Enables three-dimensional visualization, localization, and quantitative analysis of reconstructed tissue datasets.
  
  Application Areas
  Polymer Materials: Investigation of rapid crystallization, melting, glass transitions, and the effects of thermal history on material properties.
  Metal Materials: Study of melting, crystallization, and phase-transition behavior under rapid heating and cooling conditions.
  3D Printing Materials: Real-time investigation of laser-induced heating and cooling of 3D-printing powders.
  Nanomaterials: Characterization of thermal properties and stability of thin films, nanoparticles, and other microscale materials.
  Pharmaceutical Materials: Investigation of thermal behavior, crystallization kinetics, and the influence of processing conditions on pharmaceutical materials.
  Crystallization Kinetics: Study of crystal formation and growth rates under different heating and cooling conditions.
  Phase Transitions: Investigation of material-state changes, such as transitions from solid to liquid.
  Material Thermal Stability: Analysis of material degradation and decomposition behavior at elevated temperatures.

• 详细参数

  Product Specifications
  Product Type: Automated Serial Two-Photon 3D Whole-Tissue Imaging System
  Imaging Technology: Serial Two-Photon Tomography Plus (STP+)
  Imaging Modality: High-speed multi-foci two-photon fluorescence imaging
  Sample Type: Fixed intact organs and large-volume tissue specimens, particularly tissues from small animal models such as mice and rats
  Spatial Resolution: Sub-micron in-plane (XY) resolution; representative mouse-brain datasets can reach approximately 1.2 µm XY sampling
  Z-Axis Sampling: Flexible coronal or sagittal section spacing according to experimental requirements
  Fluorescence Imaging: Multichannel and multispectral fluorescence imaging
  Sample Preparation: Simple fixation and agar embedding; no optical clearing required
  Sectioning Technology: 4th-generation FlexureSlice precision microtome
  Serial Sectioning: Automated continuous tissue sectioning and imaging
  Section Preservation: Imaged tissue sections can be collected for subsequent IHC, histology, and spatial biology analysis
  Automated Slide Mounting: The TissueCyte 1600FC integrated section-capture system supports a standard capacity of 300 glass slides
  3D Reconstruction: Sequential images are registered, stitched, and reconstructed into complete 3D tissue datasets
  Data Output: High-resolution multichannel 2D images and volumetric 3D datasets
  Imaging Scale: From tissue regions to complete organs
  Operation: Designed for continuous automated operation and multi-sample studies
  Imaging Speed: Whole-tissue imaging can be completed on a timescale of hours, depending on tissue size, sampling interval, and imaging settings.

• 产品特色

  因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。

• 详细介绍

  AutoCal系统提供在线的机器人校准方案，旨在快速和自动地保证机械设备的工作性能。因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。这意味着不用猜测哪里会出错，不用浪费宝贵时间在机器人程序重复校准上，产品品质无任何损失。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。